Additional oligos什么意思
WebJan 14, 2014 · IDT has tested the stability of oligonucleotides following various storage conditions. The results of these studies indicate that the most stable temperature at which to store a standard DNA oligonucleotide is –20°C. An oligo stored at –20°C is stable for at least 24 months when either dried down or resuspended in TE buffer or nuclease ... http://www.ichacha.net/oligos.html
Additional oligos什么意思
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WebPre-designed primers. In addition to our custom synthesis options for oligos, primers, probes, and genes, we offer random primers, Oligo (dT) primers, 5’ labeled primers, gene specific primers for sequencing, forward and reverse sequencing primers and kits. Order now. Value Oligo formats. Pay per oligo, 25 and 50 nmol synthesis scales, up to ... WebNov 17, 2008 · Oligo DNA/RNA就是寡聚核苷酸的英文写法。. 通常为十几到几十个核苷酸。. 比如说作为PCR引物，通常为20个核苷酸左右的寡聚核苷酸。. 而在cDNA第一链合成过程中，通常采用oligo (dT)作为引物. Oligo DNA/RNA are short segments of nucleic acids, typically around 20nt. They can be used as ...
WebNov 14, 2024 · Tweet. #2. 02-13-2015, 06:24 AM. After the synthesis the Tn5 transposase is mixed with 2 different indexed oligos (N5xx and N7xx if you use the Illumina oligos). On average, 50% of the molecules (monomers) will carry the N7xx and 50% the N5xx index. But the transposase works as a dimer and the dimers are formed in solution in the presence … WebProduct name Custom DNA and RNA Oligos: Product Number Brand : OLIGO Sigma ... packing slip for additional terms and conditions of sale. Preparation Information Sigma-Aldrich Corporation Product Safety – Americas Region 1-800-521-8956 Version: 5.0 Revision Date: 08/16/2016 Print Date: 08/16/2016 .
WebAnneal oligos: The oligos should be resuspended in annealing buffer (10mM Tris, pH7.5-8.0, 50mM NaCL, 1mM EDTA) and mixed in equimolar concentrations. We recommend mixing 2μg each in a total volume of … Webadditional move 附加举措 ; 附带加之动作 ; 详细翻译. transshipment additional 转船附加费 ; 详细翻译. additional protocol 附加议定书 ; 增订议定书 ; 附加定议书. Additional activities …
WebJan 14, 2014 · Approximately 50% of the time (due to the 2 resulting stereoisomers that can form), PS modification renders the internucleotide linkage more resistant to nuclease degradation. Therefore, IDT recommends including at least 3 PS bonds at the 5′ and 3′ oligonucleotide ends to inhibit exonuclease degradation. Including PS bonds throughout …
Webcentrifuge dried oligos and resuspend in nuclease-free duplex buffer (100 mM Potassium Acetate, 30 mM HEPES pH 7.5) to the desired concentration (10 -100 μM). Mix the two oligo strands together in equal molar amounts, heat the oligos to 94°C for 2 minutes in a heating block or water bath and vortex; gradually cool at room temperature. small food warmerWebMar 8, 2016 · additional content的中文翻译. additional content. 附加内容;额外内容; 补充内容. 双语例句. 1. Additional content is loaded dynamically, but only when it's needed. 额外的内容只在需要时动态地装载。. 2. This provides the ability to … small food waste shredderWebA,C,T,G就是DNA的四种脱氧核糖核酸的四种碱基部分,C是胞嘧啶,dC表示的是胞嘧啶与脱氧核糖结合形成的脱氧核糖核苷酸,oligo dC则表示由胞嘧啶组成的脱氧寡核苷酸链, (oligo是“少“的意思,即只含有几个碱基的短链),ddC表示的是胞嘧啶与双脱氧核糖结合形成的双脱氧 ... small food vacuole