WebHome Restriction Endonucleases Products BsmBI-v2 BsmBI-v2 This product replaces BsmBI (NEB #R0580 ). Learn about Ligase Fidelity and Push the Limits of Golden Gate Assembly (50+ fragments). We are excited to announce … WebRestriction enzyme reaction conditions: 65 o C. Compatible ends (at this website): Asu II, Bpu 14I, Bsp 119I, Bsp T104I, Csp 45I, Nsp V, Sfu I. Heat Denaturation: BstBI cannot …
BstBI - BIOKÉ
WebpFastBac1-C-EGFP，全称为pFastBac1-PreScission-C-EGFP-His-tag-Strep-tag，是碧云天自行研发的以pFastBac1为基础构建的用于昆虫细胞表达目的蛋白C端融合有EGFP蛋白的质粒。本质粒含有polyhedrin (PH)启动子，可以高效启动目 Web100% activity in rCutSmart ™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests; Requires two or more sites for cleavage. Please review the enzyme list and recommendations in the following table as well as the background information article.; Restriction Enzyme Cut Site: GCC/GGC check safelink minutes balance
New England Biolabs (UK) Ltd - BstBI
WebAn E. coli strain that carries the cloned BstBI gene from Properties & Usage Unit Definition One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 65°C in a total reaction volume of 50 µl. Reaction Conditions 1X rCutSmart™ Buffer Incubate at 65°C 1X rCutSmart™ Buffer 50 mM Potassium Acetate 20 mM Tris-acetate WebWe are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2024 to buffers containing … WebGerman Pf 'S Publication In Insect Biochem Mol Biol.... Sites For Cloning Into The Pdonr&Trade; 221 Entry Vector. This Construct Was Then Synthesised By Genscript Corporation In Puc57 And Subcloned Into The Pdonr&Trade; 221 Entry Vector. Bstbi Restriction Enzyme Sites At The 5' End And ... check safe login