WebNov 3, 2016 · Crystal soaking tends to be experimentally simpler, ... The solvent-tolerance screen provides a means to detect problems in the soaking protocol in a systematic … WebApr 1, 2024 · Herein, a new related soaking development, the Aerosol-Generator, is introduced. This device delivers compounds onto the solution-free surface of protein crystals using an ultrasonic technique. The produced aerosol stream enables an easier and more accurate control of solution volumes, reduced crystal handling, and crystal-size …
Obtaining Crystals of Nucleic Acids in Complex with the ... - Springer
WebOct 28, 2024 · A short soak time can minimize or prevent crystal damage. Larger crystals can equal longer soak times can equal higher risk for crystal damage. Recommended reading: Guidelines for the successful … Websoaking protocols. Soaking experiments are typically undertaken when brief washes fail to provide sufficient cryopreservation of the crystal. To begin, place the crystal in the … ontrack precision centre
In-plate protein crystallization, in situ ligand soaking and …
WebJan 4, 2010 · Extensive crystal-soaking protocols have been developed to flush the bound citrate out of the active-site pocket of both molecules and the crystal structure shows that the unliganded open conformation of the A-TIM active site is the same as in unliganded wild-type TIM. It is also shown that sulfonate compounds corresponding to the transition ... WebJan 1, 2011 · In order to allow for this ∼10-fold dilution in the crystal soaking mother liquor, and a further 3–10-fold dilution factor from soaking compounds in mixtures, the compound concentration in the original stock solution must be relatively high. ... In some “heroic” cases reasonably reliable soak-out protocols have succeeded in enabling a ... WebOct 8, 2024 · Two protocols are commonly applied to produce protein-ligand complexes: cocrystallization and soaking. Because of its time and cost effectiveness, soaking is the more popular method. iot and predictive maintenance