Daughterless-gal4
http://www.girldress4less.com/ WebApr 1, 2010 · Second, we used a patchy Daughterless Gal4 driver to label a subset of epithelial cells with GFP:Moe (Fig. 1L-L″). In both experiments, similar apical, intermediate and basal-level protrusions rich in actin filaments were evident in labelled cells irrespective of the GFP fusion protein used (GFP:Moe, Pon:GFP, PA-GFP:Moe), the level of ...
Daughterless-gal4
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WebThe GAL4/UAS system is commonly used in Drosophila to drive expression of a gene of interest (Brand & Perrimon, 1993). Tissue-specific or ubiquitous enhancers drive … http://d-scholarship.pitt.edu/20724/1/ChiuWK_ETD_Final.pdf
WebMar 7, 2007 · Ubiquitous expression was obtained with the daughterless da-GAL4 line and the pan-neural expression with the elav c155-GAL4 line (Lin and Goodman, 1994). Expression in adult neurons in the pan-neural inducible line (elavGS), derived from the elavGS-301.2 line described in the GeneSwitch system ... Webda-Gal4, DaGal4, daughterless-Gal4, da::Gal4, daughterless:Gal4. Size Expression Data Associated insertion(s) 0 available. Molecular map Description and Uses Construct …
WebFor some studies, daughterless-GAL4 was combined with UAS-Dcr2 for increased RNAi efficiency . The 4e-bp RNAi (#100739 from the Vienna Drosophila RNAi Center) is … WebAug 29, 2016 · The GAL4-UAS system, developed by Brand and Perrimon to study development [6] has two components: (i) the yeast transcription factor GAL4 that is placed under the control of either a ubiquitous or tissue-specific promoter, and (ii) a gene of interest cloned downstream of a UAS sequence.
WebOct 3, 2011 · No mutant phenotypes were detected when the 8 alanine substitution mutants were expressed using the GAL4-UAS system driven by the ubiquitously expressed daughterless promoter at 29 °C. However, the C terminus did cause a nonlethal mutant phenotype under similar conditions with an apparent failure of abdominal cuticle …
Webda-GAL4 (genotype: w1118; P{da-GAL4.w-}3)—Expresses GAL4 ubiquitously and strongly under the control of daughterless 67. This driver expresses throughout development … northgate construction mnWebApr 23, 2013 · Expressing CGG 90 in the retina using GMR-GAL4 drivers severely disrupted eye morphology as characterized by loss of pigmentation, neuron death (escharosis), retinal collapse, and ommatidial fusion. Expressing CGG 90 ubiquitously using Daughterless-GAL4 drivers shortened lifespan. northgate construction cantonWebAug 29, 2016 · Using the global inducible daughterless-GS-GAL4 line, we investigated the effects of bmm overexpression specifically in adulthood by feeding the flies RU486 after eclosion. ... Systemic... northgate contractorsWebNational Center for Biotechnology Information northgate construction michiganWebAug 21, 2024 · Ubiquitous 4e-bp knockdown using the daughterless-GAL4 promoter reduced 4e-bp transcript levels (Fig. S2A) and also enhanced the effect of cold on survival (Fig. 2 B and C), although a more dramatic temperature challenge (from 18 to 29 °C) was required to reveal the phenotype, possibly due to RNAi affecting 4E-BP levels more … northgate construction servicesWebThe overarching goal of this dissertation is to import nucleus-encoded ATP6 protein into the mitochondria.This work examines the efficacies of multiple strategies in enhancing the functional outcomes of the first animal model with a stable and endogenous ATP6 mutation and shows that algal ATP6 protein provides the most promising rescue results. how to say choledochoceleWebDec 1, 2002 · The daughterless-GAL4 (Giebel and Campos-Ortega, 1997) and EP1624 (Pvf1 1624) lines were obtained from the Drosophila stock center (Bloomington, IN). The e33C-GAL4 line (Harrison et al., 1995) was a gift from C. Dearolf (Boston, MA). Pvf1, Pvf2 and Pvr cDNAs and PBc2195, XPd2444 and PBc6947 strains were from Dr Felix Karim … how to say chocolate in swahili