Dq-ova
Web（DQ-OVA是OVA与BODIPY荧光染料（DQ）共轭，并保持自猝灭状态，直到蛋白水解处理OVA生成DQ-绿色荧光为止，DQ-绿色荧光通常用于评估Ag的加工。 ） FigS18B和C可以看出小鼠吸入AF647-OVA+DQ-OVA+PS-GAMP后，AF647+DC11b+ DCs细胞明显增多。 FigS18D和E的结果表明Ag特异性CD11b + DC的增加不是由于Ag加工或吸收的改变而引 … Web1 apr 2024 · The model antigen DQ-Ova, which fluoresces upon processing by proteases inside the cell, was used to assess phagocytic capacity of the cells. Macrophages were pre-treated with 2DG (25 mM), 3PO (10 µM), or cytochalasin B (5 µm) for 45 mins prior to being cultured with complete RPMI containing DQ-Ovalbumin (500 ng/ml; Invitrogen) for 20 …
Dq-ova
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Web12 apr 2024 · Listen to your favorite songs from Angels Ova East by Chris Cassius Now. Stream ad-free with Amazon Music Unlimited on mobile, desktop, and tablet. Download our mobile app now. Web17 nov 2024 · Furthermore, OVA was by trend decreased in the presence of IL-4, which was not significant for OVA Alexa Fluor 555, but for commercially labeled OVA, DQ™ ovalbumin conjugated to BODIPY® FL dye, to detect processed antigen and Alexa Fluor 647-Ovalbumin conjugate, where ODN 2006 and IL-4 had strong inhibitory effects (Additional …
Web11 mag 2015 · DQ-OVA is minimally fluorescent while OVA is intact, but upon proteolytic processing of OVA, the fluorophore is unquenched, resulting in a large increase in fluorescence. Cell culture media on the cells was replaced with fresh TC-1 media, and the NE-OVA solution was then added to the media to obtain final concentrations of 0.025%, … WebMinimal uptake of DQ-OVA was detected at 4°C (C) . Representative images are shown from three experiments. Quantification was done as in Methods with at least 16–25 ROIs …
Web$1,392.00 / Each Description DQ ovalbumin is a fluorogenic substrate for proteases. A strong fluorescence quenching effect is observed when proteins are heavily labeled with … WebDQ OVA coupled to liposome was cultured for 15 to 60 min with macrophage clone No. 39, and FACS analysis was performed. Figure 2 a shows the fluorescence intensity of macrophages.
Web26 ago 2024 · Wnt5A mediated DQ-OVA processing is associated with the proteasome regulator PA28α-PA28β: Confocal microscopy (A, B) along with Mander’s overlap coefficient analysis (C, D) n = 2, 6-14 fields, each dot representing a field and ImageJ intensity measurement (E, F) n = 3, 7-8 fields, each dot representing a cell, demonstrate a …
WebDQ ovalbumin is supplied lyophilized in five separate vials, each containing 1 mg of conjugate. Storage and Handling Upon receipt, this product should be stored desiccated … suzuki k90 specsWeb31 mar 2016 · For in vivo DQ-OVA labeing, mice were injected intradermally into one ear with 20μl of 1mg/ml DQ-OVA while the other ear was left untreated. 24 hours later, mice were euthanized, ears were collected and single cell suspensions of dermis and epidermis were prepared as described above for analysis by flow cytometry. suzuki k90Web21 apr 2015 · DQ-OVA is a self-quenched conjugate of OVA that exhibits bright, photostable, and pH insensitive green fluorescence upon proteolytic degradation (DQ … barndiplom