Fitc sirna
WebNov 1, 2024 · Short interfering RNA (siRNA) possesses special ability of silencing specific gene. To increase siRNA stability, transportation and its uptake by tumor cells, effective delivery to the appropriate ... WebMay 22, 2005 · FITC-siRNA is introduced into both cell lines by transfection. (b) Delivery of Ku70-siRNA by ML39 scFv-P reduces Ku70 expression only in ErbB2 + cells. In the flow plots, the white histogram ...
Fitc sirna
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WebSep 17, 2024 · The siRNA, CHC/siRNA, and CHCE/siRNA nanoparticles (The siRNA was labeled with FITC) were injected into the body at the same siRNA dose per nude mouse through the tail vein. Blood samples were collected from the venous plexus of the naked eye of the mouse at the 0 h, 4 h, 8 h, 12 h, 16 h, 20 h, and 24 h time points and placed in … WebOct 31, 2024 · The FITC-siRNA hybrids with sense-strand modifiers enabled efficient mRNA knockdown (∼50-90%), which also translated to increased cell death (∼20-95%) in a …
WebOct 10, 2016 · The use of small interference RNA (siRNA) to target oncogenes is a promising treatment approach for cancer. However, siRNA cancer therapies are hindered by poor delivery of siRNA to cancer cells. Transferrin receptor (TfR) is overexpressed in many types of tumor cells and therefore is a potential target for the selective delivery of siRNA … WebSep 17, 2009 · C, binding of FITC-labeled siRNA to MCF-7 cells in the presence of C9 (black filled peak) or as a complex with C9-P (open grey peak). Cells were incubated on ice with a 4:1 mixture of FITC-labeled siRNA and C9 or C9-P for 1 h. After repeated washing, cell binding was determined by flow cytometry analysis.
WebFigure 1. Labeled siRNA is Functional. A siRNA against the 3' UTR of c-myc was labeled using the Silencer siRNA Labeling Kit (Ambion, Inc.) with Cy3 and FAM on one or both … WebApr 16, 2015 · Chondrocyte-targeting ability was also determined by fluorescein isothiocyanate (FITC)-labeled siRNA tracking under a confocal microscope. OA model was established by surgically destabilizing the ...
WebApr 11, 2024 · Similarly, 4T1 cells were incubated with [email protected] FITC and HPB-S-PP/siRNA FAM for different times to investigate the co-location of LOx FITC or siRNA FAM with lysosome. The cellular uptake of co-delivery NPs was quantitatively analyzed by flow cytometry (Beckman Coulter). Briefly, 4T1 cells were seeded in a six-well plate ...
WebOct 29, 2024 · After incubated with TIR@FITC-Nrf2-siRNA at the siRNA concentration of 1.5 µg/mL for 2 and 4 h, the cells were processed stained with LysoTracker Red and Hoechst 33342 for 20 min one by one. Finally, the cells were washed with PBS and then imaged by confocal microscopy to observe the fluorescence of lysosomes and FITC-Nrf2 … small back house for rent rancho cucamongaWebApr 21, 2024 · AuNP-PEI-peptide-FITC could bind siRNA with PEI by electrostatic interactions to form the AuNP-PEI-peptide-FITC/siRNA nanocomplexes, allowing efficient siRNA delivery in hMSCs. The adipogenic-related gene peroxisome proliferator-activated receptor γ (PPARγ) was targeted for silencing by the AuNP-PEI-peptide-FITC/siRNA … small back house for rent temeculahttp://www.qiyuebio.com/details/29626 solidworks show dangling dimensionsWebSep 17, 2009 · C, binding of FITC-labeled siRNA to MCF-7 cells in the presence of C9 (black filled peak) or as a complex with C9-P (open grey peak). Cells were incubated on … small backless bookshelfWebFigure Legend Snippet: Successful delivery of siRNA into hippocampal CA1 subfield. Fluorescent double staining of FITC-siRNA (green) and DAPI (blue) were observed in the hippocampal CA1 subfield 24 h after injection of 400 nl FITC-siRNA (10 μM), which was distributed in the cytosol of hippocampal CA1 in both sham-control ( a ) and in the rats … solidworks showing mod diamWebFITC-labeled AM and unlabeled AM were synthesized according to the sequencing results and detected by flow cytometry and laser confocal microscope. Preparation of nano-zinc … solidworks show cosmetic threads in assemblyWebOct 22, 2024 · High levels of FITC fluorescence derived from siRNA internalization were detected only in CXCR4 + Jurkat T cells when incubated with 4M5.3X4 + siRNA conjugate (Fig. 2a), even surpassing transfection control (fold-increase of mean fluorescence intensity was ~ 4 for 4M5.3X4 + siRNA comparing with transfected siRNA; Fig. 2a). From flow … small backless bench