Irf8 flox mouse
WebUpon arrival, some Irf8 f mice were bred with B6.Cg-Tg(Sox2-cre)1Amc/J mice (Stock No. 008454) for germline removal of floxed exon 2. Offspring with pan deletion of floxed exon … WebMyeloid cell-specific IRF8 conditional knockout mice (IRF8flox/flox; Lyz2Cre/+) with a C57BL/6 background were generated by mating IRF8-flox mice (Feng et al. 2011) with Lyz2-Cre knock-in mice. The primers used for genotyping are shown in Supplementary Table S3. The genotyping results are shown in Supplementary Figure S4a. Harvesting of blood ...
Irf8 flox mouse
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WebIRF8 and IRF1 are transcriptional regulators that play critical roles in the development and function of myeloid cells, including activation of macrophages by proinflammatory signals … WebProduct Specific Information. Description: The V3GYWCH monoclonal antibody reacts with human and mouse interferon regulatory factor 8 (IRF8; ICSBP). IRF8 is a 50 kDa transcription factor that plays a critical role in the development of dendritic cells (DC). Specifically, IRF8 is essential in the development and differentiation of plasmacytoid ...
WebJan 1, 2014 · Here we show that IRF8 is downregulated in both mouse hearts and cardiomyocytes after hypertrophic stress treatment. Moreover, IRF8-deficient mice reveal an aggravated pressure overload-induced hypertrophic response, whereas the opposite effects occur in IRF8-overexpressing mice. WebCreLysMFasflox/flox mice expressed Fas on B and T lym-phocytes isolated from peripheral blood (ref. 10 and data not shown) and the peritoneal cavity (Figure 1A). In contrast, Fas …
WebDuring our analyses, we also examined littermate mice with a genotype of floxed IRF8 only, PU.1 only, or IRF8/PU.1 heterozygous with or without Mb1-Cre (SI Appendix, Table S1). While Mb1-Cre–mediated PU.1 deletion alone did not affect B cell distribution in the BM, Mb1-Cre–mediated IRF8 deletion alone appeared to induce a moderate reduction ... WebMar 8, 2024 · SSc remain unknown. IRF8 is a transcriptional regulator that is essential for the differentiation and function of monocytes and macrophages and thus may be involved in the regulation of macrophage phenotypes in SSc fibrosis. In this study, we measured IRF8 levels in circulating monocytes of 26 patients with SSc (diffuse cutaneous SSc,
WebFigure Legend Snippet: Upregulation of downstream targets of ERK pathway. A. Western Blot analysis of protein extract from normal appearing mucosa obtained from small intestines tissues from LSL-K-RasG12D and Villin-Cre/LSL-K-RasG12D mice. 10 ug of total protein extracts from LSL-K-RasG12D and Villin-Cre/LSL-K-RasG12D were analyzed by Western …
WebDec 11, 2024 · Mice with IRF8 deficiency only in colon epithelial cells (termed IRF8cKO) were created by crossing Irf8-floxed mice with villin-cre mice (Figures S1A and S1B) with the deletion of exon 2 (Figure S1C). As reported in the literature, we observed that IRF8KO mice exhibit elevated levels of CD11b + Gr1 + MDSCs as compared to wild-type (WT) mice. … screwball assesWebThe Transgenic and Knockout Mouse Shared Resource provides members of the DCI with comprehensive and user-friendly services for the production of custom designed, … pay boston excise tax onlineWebJun 21, 2024 · We generated a mouse line with Irf8 -deficiency in Clec9a -expressing progenitors and hypothesized that Irf8 -deficiency would deplete cDC1s, which may promote a pro-inflammatory immune response and consequently drive AKI/AKD progression. Materials and Methods Animals pay bowflex