WebDissolve the amino-modified oligo in 125μl of conjugation buffer. Add 6μl of TAMRA/DMSO solution (ca. 5-fold excess). Vortex the mixture and incubate at 37°C in the dark overnight. Separate the oligo-TAMRA conjugate from salts and free TAMRA by size-exclusion chromatography on a NAP-10 column or equivalent. Web29 dic 2000 · 5-Carboxy Tamra 1 was conjugated to 4-hydroxypiperidine with BOP and N-methylmorpholine, ... (Tamra) phosphoramidite facilitates solid-phase-supported synthesis of 5'-Tamra DNA J Org Chem. 2000 Dec 29;65(26):9033-8. doi: 10.1021/jo0011134. Authors M H Lyttle 1 , T G Carter, D J Dick, R M Cook. Affiliation 1 …
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Web15 ago 2024 · Intracellular TAMRA fluorescence also perfectly overlapped with UCNPs luminance at 450 nm in CLSM images, indicating the integrity of DNA nanomachine during internalization process (Fig. 4 A). Colocalization experiment was performed with DNA nanomachine-FITC prepared with substrate S-SH’ in the absence of BHQ1, which … Web2. Detailed DNA and RNA sequences used in this work. 3. The excitation and emission wavelengths of the fluorophores. 4. The size distribution of PDA-PEI nanodots 5. The stability of PDA-PEI nanodots. 6. Relationship between the QE and the concentration of TAMRA-DNA. 7. Comparison of QE with the previously reported quenchers. 8. primary franchise vs secondary franchise
Dual Labeled Fluorescent Probes: TAMRA Quencher
http://genelink.com/newsite/products/mod_detail.asp?modid=37 Web12 ott 2024 · Fluorophore-linked, sequence-specific DNA binding reagents can visualize … WebThe unique characteristics of the BHQ™ variants permit flexibility in the choice of spectrally well-resolved reporters, which enable single-tube multiplexing with little or no cross-talk. In summary, Black Hole Quencher™: Has no native fluorescence (emits heat instead of light), resulting in lower background fluorescence. played daphne moon in frasier jane